2021 Fall - UH - Mon - Alice Vu

Soil sample obtained on 08/29/2021 at Bishop Fiorenza Park nearby a tree.

Inoculated tubes observations:

Week 0: Brown, muddy.

Week 1: Colors lightened up. Lighter colors on SSM compared to CSM.

Week 2: 3/4 tubes are clear-colored except for Px on CSM. Yellow color indicates possible OP hydrolysis.

Week 3: Tubes on SSM are clear-colored. Px on CSM is yellow-colored and also Px on SSM (lighter yellow shade). Indicates OP hydrolysis

Week 4: Similar results to week 3

Week 5: 4/4 tubes show clear color. Slight cloudiness in previously yellow-colored tubes (Px on CSM and SSM).

Px Plate: No halos around colonies, thus no signs of OP hydrolysis seen. Bacteria able to tolerate OP pesticide but not degrade it.