My soil sample was isolated from a farm in Scottsville. Growth of soil bacteria was done for 2 weeks. Isolation of soil bacteria lasted for 5 weeks in CSM Mp, SSM Mp, CSM Px, and SSM Px. The liquid cultures were then plated on Mp and Px plates to grow single colonies. The single colonies were then picked with a toothpick and inoculated onto an Mp or Px plate. The isolated bacteria were then picked with a toothpick and mixed with 100 microliter of water. The mixture was then heated at 95 degrees C for 6 minutes before it was sent off for sequencing. The sequences were given back to me and input into BLAST to find the genus and species of bacteria that were isolated. MR#1: Bacteria: Enterobacter hormaechei MR#2: Bacteria: Enterobacter cloacae or Klebsiella pneumoniae MR#3: Bacteria: Raoultella planticola This bacteria was formerly part of the Klebsiella genus. It is also used in bioremediation of cadmium from exposed soils. The bacteria is often found in soil and was attempted to be bioengineered and used in farm soil to destroy crop residues and produce ethanol. MR#4: Bacteria: Rosenbergiella nectarea This bacteria was isolated from the flower nectar of almonds and grapefruit, then sequenced. All photos: https://1drv.ms/a/s!Avq4IFiAJyuEgbx-cVeh337uyJrkNQ
