2021 Spring | UH | Wed | Phu Huynh |

Overview
Sample Description

Week 0: Soil was collected during the afternoon around 05:15 PM. The weather was partly cloudy with a moderate air quality (60), 20% chance of rain, 58% humidity, 75°F in temperature, the pressure of 29.79 mmHg, SW wind of 60 mph. The location of the collection was Channelview, TX 7753. 

1 g of soil sample was measured and inoculated into an 8 mL LB media in an empty 14 mL culture tube. The tube was inoculated for a week at 30°С and 200 rpm. 

Observation: The tube was brown and opaque with black precipitate at the bottom of the tube.

Week 1Four tubes were labeled CSM+Px, CSM+MP, SSM+Px, and SSM+MP. In CSM+Px tube, 1 mL of soil inoculate and 4 mL minimal media CSM with 5µL Paraoxon. In CSM+MP, 1 mL of soil inoculate and 4 mL minimal media CSM with 5µL Methyl Parathion. In SSM+Px tube, 1 mL of soil inoculate and 4 mL minimal media SSM with 5µL Paraoxon. In SSM+MP tube, 1 mL of soil inoculate and 4 mL minimal media SSM with 5µL Methyl Parathion. Four tubes were incubated at 30°С and 120 rpm for 1 week.

Observation: The solution in the SSM+MP tube was slightly opaque with a pale yellow color. The solution in the CSM+MP tube was clear with a pale yellow color. The solution in the SSM+Px tube was opaque with a dark yellow/orange color. The solution in the CSM+Px tube was clear with a pale yellow color.

Week 2:  Four tubes were labeled as done the previous week. In each tube, 5 mL of media with 5 µL pesticide were added respectively to the name of the tube. In each tube, 200 µL of the previous week's inoculation were added aseptically to the corresponding current week's tube. The tubes were incubated at 30°C and 200 rpm for one week.

Observation: The solution in the SSM+MP tube was clear with no color. The solution in the CSM+MP tube was clear with no color. The solution in the SSM+Px tube was clear with a pale yellow color. The solution in the CSM+Px tube was clear with a pale yellow color.

Week 3:  Four tubes were labeled as done the previous week. In each tube, 5 mL of media with 5 µL pesticide were added respectively to the name of the tube. In each tube, 200 µL of the previous week's inoculation were added aseptically to the corresponding current week's tube. The tubes were incubated at 30°C and 200 rpm for one week.

Observation: The solution in the SSM+MP tube was clear with no color. The solution in the CSM+MP tube was clear with no color. The solution in the SSM+Px tube was clear with no color. The solution in the CSM+Px tube was clear with a pale yellow color.

Week 4:  Four tubes were labeled as done the previous week. In each tube, 5 mL of media with 5 µL pesticide were added respectively to the name of the tube. In each tube, 200 µL of the previous week's inoculation were added aseptically to the corresponding current week's tube. The tubes were incubated at 30°C and 200 rpm for one week.

Observation: The solution in the SSM+MP tube was clear with no color. The solution in the CSM+MP tube was clear with no color. The solution in the SSM+Px tube was clear with no color. The solution in the CSM+Px tube was clear with a pale yellow color.

Week 5:  Four tubes were labeled as done the previous week. In each tube, 5 mL of media with 5 µL pesticide were added respectively to the name of the tube. In each tube, 200 µL of the previous week's inoculation were added aseptically to the corresponding current week's tube. The tubes were incubated at 30°C and 200 rpm for one week.

Observation: The solution in the SSM+MP tube was clear with no color. The solution in the CSM+MP tube was clear with no color. The solution in the SSM+Px tube was clear with no color. The solution in the CSM+Px tube was clear with a pale yellow color.

Researcher's Name

Date of Collection
Semester of Collection
Fall 2020
My Institution
UH
Results
Number of Colonies of Paraoxon
12
Number of Colonies of Methyl Parathion
0
Location
Geolocation for Sample
29.792555, -95.07174
Location Name
Channelview
Location Street
Market St.
Location City
Channelview
Location County
Harris
Location State
Texas
Location Country
US
Location Postal Code
77530
My Lab Section
Wednesday

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