Soil Sensing Map

Sample #24; Under a tree; Southwyck Parkway, Pearland, TX 77584 Brazoria County

Sample #23; CR 511, Rosharon, TX 77583 Brazoria County

Sample #22; Fallow farmland; CR 172, Rosharon, TX 77583 Brazoria County

Sample #21; CR 511, Rosharon, TX 77583 Brazoria County

Sample #20; Along bayou; Bayou Dr., Alvin, TX 77511 Brazoria County

Sample #19; By a school; Greenbriar Dr., Friendswood, TX 77546 Galveston County

Sample #18; Maintained landscaped lot; West Blvd., Friendswood, TX 77546 Galveston County

Sample #17; By a creek; Briar Bend Dr., Friendswood, TX 77546 Galveston County

Sample #16; Active farmland; CR 169, Alvin, TX 77511 Brazoria County

Sample #15; Fallow farmland; CR 172, Rosharon, TX 77583 Brazoria County

Sample #14; Farmland; CR 212, Angleton, TX 77515 Brazoria County

Sample #13; By a park; Shadwell Ln., Friendswood, TX 77546 Galveston County

Sample #12; By a lake; Trail (no street); Friendswood, TX 77546 Galveston County

Sample #11; Westglen Dr., Alvin, TX 77511 Brazoria County

Sample #10; By a park; Fife Ln., Webster, TX 77598 Harris County

Sample #9; CR 511, Rosharon, TX 77583 Brazoria County

Sample #8; Bayou Dr., Alvin, TX 77511 Brazoria County

Sample #7; Fallow farmland, CR 172, Rosharon, TX 77583 Brazoria County

Sample #6; CR 511, Rosharon, TX 77583 Brazoria County

Sample #5; ACC Campus by greenhouse; Mustang Rd., Alvin, TX 77511 Brazoria County

Sample #4; By a baseball complex; Stadium Ln., Friendswood, TX 77546 Galveston County

Sample #3; By industrial shop; Fox Meadow Dr., Alvin, TX 77511 Brazoria County

Sample #2; Edge of Splash park at city park; McLean Rd., Pearland, TX 77584 Brazoria County

Sample #1; By a park; Bonanza Rd., Houston, TX 77062, Harris County 

I collected soil from my apartment complex. I did the soil that was furthest from the road so that there was not any of the chemicals from that. The pesticides known to have been used here are Baricor sp and demand.

Collected at a steep Brays Bayou tributary channel that run-offs from nearby home gardens usually cover.

Sample collection site was in a drainage ditch near the water.

 Soil sample collected from 37.6615 latitudes.   -113.0778 Longitude, name of the insecticide/pesticide used: home defense pesticide. collected from a garden in front of the house.

This sample was collected from a water drainage stream near a neighborhood that has seen consistent use of pesticides to avoid infestations.

dirt collected around the base of the tree that is sprayed each season 

Bacteria from soil sample collected and later plated from Paraoxon subculture was most likely from genus Pseudomonas and appeared to have clearing or halos around bacterial colonies indicating hydrolysis of Paraoxon Pesticide.

Soil sample from my front yard in Syracuse NY

Soil sample comes from an alfalfa field that was recently sprayed in May 2020 for Alfalfa Weevil, Hypera Postica. Sample was collected using a flat headed screwdriver to pry up a clod of dry soil about the size of a tennis ball. The sample was then promptly placed into a quart sized ziplock back and sealed.  Lambda-cyhalothrin and Chlorpyrifos were the pesticides sprayed in May 2020. The owner also said that they had not sprayed the field with pesticides for several years prior to May 2020.

Dirt collected around the perimeter of a house, pesticide Tempo SC Ultra Control (beta-Cyfluthrin 11.8%) has been used in 2020

From the 16s sDNA, I have concluded that this sample is Brevundimonas diminuta, which is a gram negative rod that tests positive for catalase and oxidase and is non motile. My sample has those exact characteristics

The soil was collected 8 inches deep in the ground, the sample was damp and sticky.

The soil sample was collected 6 inches under the surface of the ground and placed in an amber tube. The location was near Lubrizol and Buffalo Bayou on Tidal Rd.

Soil sample collected was at a nearby neighborhood bayou where it experiences frequent flooding, which possibly contributes to the growth of various bacteria. Additionally, this area is predicted to have come into contact with pesticides to certain plantation from growing in the area. 

The sample was collected in an area known for chemical dumping (Brio Superfund Site) until stopped by the EPA for protection of the environment.

The soil was collected near a duck pond, at 1900 on 1/28/2021. The soil sample was wet, with corn kernel visible from the duck feed.

The soil sample was collected near the Brio Superfund industrial site that was located near San Jacinto College. This site was known for storing Petroleum and waste until EPA taken it down due to leakage of toxic waste. Contaminated soils were dug out and removed.

This sample was taken from a home garden on 1/13/20

The sample was collected from a drainage ditch off of West Airport in Fort Bend County. At the time of collection, there had been a short downpour, so the soil was quite moist and soggy. 

The sample was collected, where plant growth was not much. There was some landscaping done on a non-regular basis which includes using pesticides. This place was also a place of interest as it is in very close proximity to chemical plants. 

• Bacteria for this research was collected from a home lawn with the coordinates 37.25991019552653, -113.32437110280057. This soil has been known to have Bayer Advanced 24-hour grub killer applied to it to protect the grass from insects. 

Sample of dirt collected near train tracks 

The plot I selected dirt from was the area of dirt surrounding a residential house where pesticides have been used. The pesticide used was Home Defense Ortho brand.

The sample was collected from a pond in Katy, Texas. 

Sample collected off of a near-stationary stream that often collects itself with large quantities of water full of pesticide, when, applied by park monitors. 

Sample was found outside a residence in a planter box in Cedar City UT. (37.657257, -113.073790)

Obtained near sewage drain by fishing spot.

The sample was collected near Memorial Park Conservancy. This place likely to have pesticide use or activity, because it is expected to discover organophosphate enzymes where pesticides are used, the collection of the sample was performed near an area where pesticides are ubiquitous. Following several inoculations, screening was carried out on the CMM plate to detect OP hydrolysis. The bacterial growth essentially indicated resistance to the OP compounds, whereas the “halo effect” was the evidence for OP hydrolysis. Moreover, CSM subcultures indicated a more intense color, as compared to the SSM subcultures. The findings of the Screening results indicated countless colonies with the “halo effect” on the CMM subculture. In summation, this experiment indicated a positive result, in that the collected soil possessed microorganisms that were able to express the organophosphorus hydrolase enzyme and organophosphate degradation.

The location I collected from was my backyard where my landlord regularly sprays insecticides (Ortho Home Defense) 

The sample was collected in a wooded area where a small river was flowing through and it was a mile away from a farm. For inoculation results, the inoculation samples turned yellow in minimal media subcultures containining paraoxon (Px) which indicates production of P-nitrophenol due to OP hydrolysis. The color was brighter in CSM medium compared that in SSM medium which suggest that the bacteria prefer carbon as their food source. As for subcutlures containig methyl parathion (MP), they showed no colors after a series of inoculation. For screening result, the individual colonies and halos were visible on the CMM plate. In conclusion, the soil sample contained bacteria capable of expressing organophosphorus (OP) hydrolase and breaking down OP compound.

A sample of soil was taken from a home in Southern Utah that biyearly applies Ortho Home Defence Insect Killer. Of which has the active ingredients of Bifenthren and Zeta-Cypermethrin.

Week 0: Soil was collected during the afternoon around 05:15 PM. The weather was partly cloudy with a moderate air quality (60), 20% chance of rain, 58% humidity, 75°F in temperature, the pressure of 29.79 mmHg, SW wind of 60 mph. The location of the collection was Channelview, TX 7753. 

1 g of soil sample was measured and inoculated into an 8 mL LB media in an empty 14 mL culture tube. The tube was inoculated for a week at 30°С and 200 rpm. 

Observation: The tube was brown and opaque with black precipitate at the bottom of the tube.

Week 1: Four tubes were labeled CSM+Px, CSM+MP, SSM+Px, and SSM+MP. In CSM+Px tube, 1 mL of soil inoculate and 4 mL minimal media CSM with 5µL Paraoxon. In CSM+MP, 1 mL of soil inoculate and 4 mL minimal media CSM with 5µL Methyl Parathion. In SSM+Px tube, 1 mL of soil inoculate and 4 mL minimal media SSM with 5µL Paraoxon. In SSM+MP tube, 1 mL of soil inoculate and 4 mL minimal media SSM with 5µL Methyl Parathion. Four tubes were incubated at 30°С and 120 rpm for 1 week.

Observation: The solution in the SSM+MP tube was slightly opaque with a pale yellow color. The solution in the CSM+MP tube was clear with a pale yellow color. The solution in the SSM+Px tube was opaque with a dark yellow/orange color. The solution in the CSM+Px tube was clear with a pale yellow color.

Week 2:  Four tubes were labeled as done the previous week. In each tube, 5 mL of media with 5 µL pesticide were added respectively to the name of the tube. In each tube, 200 µL of the previous week's inoculation were added aseptically to the corresponding current week's tube. The tubes were incubated at 30°C and 200 rpm for one week.

Observation: The solution in the SSM+MP tube was clear with no color. The solution in the CSM+MP tube was clear with no color. The solution in the SSM+Px tube was clear with a pale yellow color. The solution in the CSM+Px tube was clear with a pale yellow color.

Week 3:  Four tubes were labeled as done the previous week. In each tube, 5 mL of media with 5 µL pesticide were added respectively to the name of the tube. In each tube, 200 µL of the previous week's inoculation were added aseptically to the corresponding current week's tube. The tubes were incubated at 30°C and 200 rpm for one week.

Observation: The solution in the SSM+MP tube was clear with no color. The solution in the CSM+MP tube was clear with no color. The solution in the SSM+Px tube was clear with no color. The solution in the CSM+Px tube was clear with a pale yellow color.

Week 4:  Four tubes were labeled as done the previous week. In each tube, 5 mL of media with 5 µL pesticide were added respectively to the name of the tube. In each tube, 200 µL of the previous week's inoculation were added aseptically to the corresponding current week's tube. The tubes were incubated at 30°C and 200 rpm for one week.

Observation: The solution in the SSM+MP tube was clear with no color. The solution in the CSM+MP tube was clear with no color. The solution in the SSM+Px tube was clear with no color. The solution in the CSM+Px tube was clear with a pale yellow color.

Week 5:  Four tubes were labeled as done the previous week. In each tube, 5 mL of media with 5 µL pesticide were added respectively to the name of the tube. In each tube, 200 µL of the previous week's inoculation were added aseptically to the corresponding current week's tube. The tubes were incubated at 30°C and 200 rpm for one week.

Observation: The solution in the SSM+MP tube was clear with no color. The solution in the CSM+MP tube was clear with no color. The solution in the SSM+Px tube was clear with no color. The solution in the CSM+Px tube was clear with a pale yellow color.

This was near the bayou of Macgregor Parkway, a park in Houston that sees a regular treatment of pesticides to fight of weeds and other unwanted organisms. Frequent flooding in the area also helps contribute to the variety of microorganisms that may be found. 

Pesticide reported to have been used: Eight Insect Control (for Yard and Garden)

Active ingredient: Permethrin 2.50%

Site description: The site description is a home garden.

A pet-friendly apartment complex lawn. 2,4-D has been used for lawn maintenance.

Sample was taken near lake at Buffalo Run Park. Sample tested positive for OP hydrolysis for Px+CSM and Px+SSM. 

Soil sample obtained upstream Mason Creek heading towards Buffalo Bayou assumed to have pesticide runoff from surrounding neighborhoods west of Houston. The soil was very damp and yellowish indicating excess iron.

• Approx. 50g of soil sample was collected from the bottom of a hole 12in deep. The soil was placed inside an amber tube and Ziploc bag to protect it from light exposure during transportation to the lab. 
• Week 0: 1g of soil was added to 6mL of LB media and left to incubate at 30°C, 200rpm for 2 weeks (*1 additional week due to winter storm Uri). 
• Week 1: 1mL of LB culture was transferred into 4 tubes containing 4mL of CSM/SSM minimal media with Px/MP pesticide for a total of 5mL. Each tube was incubated for 1 week at 30°C, 200rpm. 
• Week 2-5: The previous week's tubes were collected and the innoculation was transferred into fresh media tubes containing 5mL of the corresponding CSM/SSM minimal media with Px/MP pesticide. Each tube was incubated for 1 week at 30°C, 120rpm. This procedure was repeated 3 times on a weekly basis. 
• Week 6: 1mL of sample is taken from the CSM Px tube displaying the strongest (+) yellow color and innoculated in 10mL of enriched media to grow overnight at 37°C, 200rpm. The innoculation is transferred to a minimal media plate with Px pesticide and grown for 7 days to observe for OP hydrolysis. 

Soil samples were taken from the lawn in front of the apartment.
 

The sample was collected 7-8 inches deep next to a bayou that usually get flooded during heavy rain. 

My soil sample was isolated from a farm in Scottsville. Growth of soil bacteria was done for 2 weeks. Isolation of soil bacteria lasted for 5 weeks in CSM Mp, SSM Mp, CSM Px, and SSM Px. The liquid cultures were then plated on Mp and Px plates to grow single colonies. The single colonies were then picked with a toothpick and inoculated onto an Mp or Px plate. The isolated bacteria were then picked with a toothpick and mixed with 100 microliter of water. The mixture was then heated at 95 degrees C for 6 minutes before it was sent off for sequencing. The sequences were given back to me and input into BLAST to find the genus and species of bacteria that were isolated. 

MR#1:

Bacteria: Enterobacter hormaechei

MR#2:  

Bacteria: Enterobacter cloacae or Klebsiella pneumoniae

MR#3: 

Bacteria: Raoultella planticola 

This bacteria was formerly part of the Klebsiella genus. It is also used in bioremediation of cadmium from exposed soils. The bacteria is often found in soil and was attempted to be bioengineered and used in farm soil to destroy crop residues and produce ethanol.  

MR#4: 

Bacteria: Rosenbergiella nectarea

This bacteria was isolated from the flower nectar of almonds and grapefruit, then sequenced. 

All photos:

https://1drv.ms/a/s!Avq4IFiAJyuEgbx-cVeh337uyJrkNQ

<p>Soil Sample taken from a residential backyard garden in Friendswood, Texas, which is maintained by the owner to grow various vegetables and spices.</p>

<p>The soil sample was taken from Froberg's Farm, where pesticides are used. The bacteria from the Week 5 CSM+Px test tube were able to tolerate Px but not degrade it. The growth on the plate was very weak, resulting in approximately 20,208 colonies.</p>

<p>McGovern Centennial Gardens at Hermann Park was chosen for soil sampling because it is a community garden in which consumable products are grown. The nature of the produce being grown and the location were also factors that were taken into consideration.</p>

<p>Soil sample from River Green Park in Katy, Texas (10 inches deep). The soil is very moisture and dark.</p>

<p>Very tiny colonies grew on the agar (~8478), but there was no evidence of Px hydrolysis based on the edges of the bacterial colonies. This means that the bacteria can tolerate the Paraoxon pesticide, but not degrade it.</p>

<p>Collected the bank of Greens Bayou near the underpass of the Green River Drive bridge. Hurricane Harvey had hit Houston recently.</p>

<p>The sample was collected from a ditch on the corner of Liberty Dr and Julie St. Mosquito trucks drive by and spray pesticide every week during the summer months.</p>

<p>The sample was taken from the soil in a front yard in South Houston. The sample tested positive for Paraoxon, producing tolerant colonies.</p>

<p>A soil sample was collected near a guava tree from a residential backyard. The sample was positive for paraoxon tolerance.</p>

<p>This sample was taken from the Walmart garden center, where they house plants and other botanical forestry</p>

<p>This garden grew crops the family ate from daily so it is very likely that they used very minimal pesticides to treat their soil. Sample was moist and dark and collected on a sunny day at approximately 11:30 AM. Sample was not further processed due to lack of evidence of strong hydrolysis.</p>

<p>Soil sample with unknown composition</p>

<p>Sample was collected after hurricane Harvey when it was still very wet.</p>

<p>Soil was collected near a sewer in a ditch behind HEB. The bacteria were found to be tolerating the paraoxon pesticide.</p>

<p>taken from bear creek park in Houston, Tx, soil taken from approximately 12 inches into the ground,</p>

<p>Sample was taken from John P. McGovern Centennial Gardens in Houston, TX.</p>

<p>The soil sample was collected from the small garden where usually use pesticide</p>

<p>This sample was collected near a drain at Cullen park which is a part of Addicks Reservoir, after Hurricane Harvey</p>

<p>sample was taken under bridge in the middle of Steep Bank Creek</p>

When collected, the soil sample had a dark muddy appearance.

<p>Soil collected from 12 inches beneath the frozen surface.&nbsp; Sample analyzed by Kevin Schutt.</p>

<p>Stopped on the side of the street near the facility, near a large&nbsp;electrical box and plenty of weeds.</p>

<p>Sample was positive for paraoxon degradation.</p>

<p>Samples pictures under "sample background".</p>

<p>&nbsp;</p>

<p>My sample contains approximately 80-95 positive tolerant colonies. The tolerant bacterial colonies weakly or incompletely degraded CSM media containing Paraoxon pesticide to grow and survive.</p>

<p><strong>Rhizobium radiobacter GHKF11&nbsp;</strong></p>

<p>Previous pesticides used were: Ortho, Flower, Fruit, and Vegetable (organic). The soil sample was from a home garden that was covered in snow and on top of cobble stone. The soil sample was very dark and rich.</p>

<p>Ladybug Nursery at&nbsp;which permethrins, pyrethrins and sulfur was used in a number of plants.&nbsp;</p>

<p>Located on operational wholesale nursery specializing in trees and shrubbery. Owners only provided name of herbicide (Round-Up) which is used twice a year.</p>

<p>Broccoli field at which&nbsp;many pesticides were used during the 2019 growing season. The pesticides used were&nbsp;Methoxyfenozide, Alcohol Ethoxylates, Thiamethoxan, Oxyfluren, Spiromesifen, Acetamiprid, Bifenthrin, Dinotefuran, Spinetoram, and Buprofesin. The previous crop was Fallow.&nbsp;</p>

<p>I collected this sample on a Sunday 2/2/2020 afternoon around 2pm. It was a clear day and the ground was just moist not too wet. This place was a previously empty land that has recently turned into an active farmland.</p>

<p>Coordinates: (29.647715, -95.716027).</p>

<p>The soil sample was collected at approximately 9AM on January 27th. The soil an its surrounding environment were wet when the sample was being collected. The collection site was at near the intersection between US-59 and US-59 BUS&nbsp;at Hungerford. There was no plants being grown on the collection site, however, there was some signs of plow and pipelines nearby the field. The sample was collected at approximately 8 inches depth of digging from the surface. This sample was collected for the testing of bacteria's pesticide degradation ability.&nbsp;</p>

<p>This location was chosen based on the fact that it was a farmland and active farming is done here in this 288 acre plot land. The location is openland across all the sides. The sample was collected from Houston outskirts, precisely from Richmond. The land soil was moist and had a dark texture. It almost looked as if new fresh batch of cultivation was seen. The location had just one Harlem road connecting it to the city. It was mostly quiet with few to none population around. The closest residential place from the location is 8 miles.There was no water body seen around. To get the sample I went inside the farmland towards the center and dug a hole. I took a scoop of soil sample. The soil sample was dark texture with very few fine particles, thick in context, had a distinct pungent smell to it.&nbsp;</p>

<p>Methyl parathion and paraoxon - negative results for all inoculation weeks. Paraoxon Methyl parathion CSM PSM SSM CSM PSM SSM Week 1 N N N N N N Week 2 W W W W W W Week 3 T T T T T T Week 4 T T T T T T Week 5 T T T T T T</p>

<p>Paraoxon (PX) Methyl Parathion (MP) CSM SSM CSM SSM Week 1 So So So So Week 2 T T T T Week 3 T T T T Week 4 T T S S Week 5 Y T S S Negative results for both Paraxoan and Methyl Parathion degrading bacteria.</p>

<p>Soil sample was collected from irrigation ditches in a flower bed.</p>

<p>Soil sample collected near the San Jacinto river.</p>

<p>Negative results for paraxoan and methyl parathion digesting bacteria.</p>

<p>The soil was light brown and dry.</p>

<p>A small soil sample was collected on a plot of farming land on Fernglade Drive near 4033 Gladeridge Dr, Houston, TX 77068.</p>

<p>29°34’8"N 95°13’28"W</p>

<p>A sample was taken from a home garden. it was then tested to see if pesticide degrading enzyme were found.</p>

<p>Muddy soil sample with unknown composition</p>

<p>sample taken from a Landscaping, Nursery and Tree farm in Pasadena Texas.</p>

Apt. garden

<p>Portico at West 8 apartments</p>

<p>Growth of pesticide resisting bacteria colonies in Paraoxon and Methyl Parathion. In Paraoxon plate degrading bacteria is present. In Methyl Parathion plate tolerating bacteria is present.</p>

<p>Park is regularly treated with pesticides and other chemical agents.</p>

<p>Soil sample collected near the Historic Santa Anna Capture Site</p>

Outside of a neighborhood, in a big field with long grass and a tunnel water pipe running through it.

Soil Sample from Keegans Bayou

<p>Soil collected from surface.&nbsp; Sample analyzed by Kara Morrison</p>

<p>Sample dug next to the pond in Meyer park.</p>

<p>The location from where the sample was taken from had previous exposure to pesticides, and was taken from the backyard of a garden in the Northwest Houston Area.</p>

GIS coordinates

<p>Home Garden at which Preen Garden Weed Preventer&nbsp;(pre-emergent) was used during the 2018 growing season.&nbsp;</p>

<p>A home garden where Sevin, which contains Carbaryl, was used in the 2018 and 2019 growing season.</p>

<p>Private greenhouse where OrthoMax was used during the 2019 growing season.&nbsp;</p>

<p>Private home greenhouse garden where quinclorac, 2,4-D, dicamba, and trifluralin have been used in the last 2-3 years.&nbsp;</p>

outside of an apartment building by a sewer grate, there has been Ortho Home Defense Pesticide used for ants around the perimeter in 2019. 

<p>Flower garden in front of house where Ecovia and Demand were used during 2019.</p>

<p>A yard near the road at which no known pesticides were used in recent seasons.&nbsp;</p>

<p>This sample was obtained at the bayou that was located at the intersection off of grandparkway (SH 99) and FM 1464 in Sugar Land, Texas.</p>

<p>Sample was taken from a small floral garden. Soil was dry, light brown in color, and full of small rocks.</p>

<p>Over the course of a few weeks, it was evident that no result was going to be obtained for MP. By the 5th week, all tubes were close to becoming clear with a very slight yellow color.</p>

<p>The location was in Buffalo Bayou between Sam Houston Tollway and Independence Pkwy. The soil was collected near the Old River along Launch Rd (Old River Terrace, Channelview TX). The approximate latitude and longitude coordinates are: 29°46'54.2"N 95°06'03.3"W. The soil was collected on August 30, 2016 around 9:30 PM. The collected sample was clay-like and moist because of the recent rainy weather.</p>

<p>Ballester Fishing Area - La Porte</p>

<p>20g of soil were obtained from Alex's garden. Soil has been exposed to pesticides for at least 12 months.</p>

<p>Paraoxon positive, MP negative</p>

<p>testing soil sample to see if it has signs of pesticide degrading bacteria in it.</p>

<p>Soil collected at a soccer field in southwest Houston.</p>

<p>Very clay like, thick and adhesive. Plenty of moisture</p>

<p>Muddy sample with unknown composition</p>

<p>Sample positive for paraoxon hydrolysis. Sample negative for methyl parathion hydrolysis</p>

<p>Dirt was retrieved on a cliff near the river, the dirt was moist since it had rained a day or two before it was collected.</p>

<p>Soil sample collection plated on CSM agar for the viewing and identification of degrading organophosphorus bacteria.</p>

<p>The sample was obtained near the Sweetwater Cypress Golf Course on September 3rd, 2012.</p>

<p>Collected in a home garden.</p>

<p>Was clumpy, wet soil from a ricefield.</p>

<p>Soil collected from Tom Bass Regional Park near Clear Creek.</p>

The collected soil sample that I have provided was obtained at the coordinates 29.7238200, -95.1137860 on August 28, 2018 at 12:41PM on a sunny day. The collected sample was taken from the East Fork Patrick Bayou nearby the Buffalo Bayou. The sample was retrieved from an 8-12 inch hole along the bank of the bayou. For week 0, 1.05g of the sample is initially inoculated into an enriched media then for week 1-5 200µL of the previous week’s inoculation culture is added to separate minimal media tubes containing Carbon Selective Media with Paraoxon, Carbon Selective Media with Methyl Parathion, Sulfur Selective Media with Paraoxon, and Sulfur Selective Media with Methyl Parathion. The best indicator for the metabolism of pesticides is then inoculated onto the enriched media for bacteria revitalization then 100μL of the enriched media with bacteria is plated onto a minimal media with MP. The plated sample is then analyzed to confirm that the bacteria is able to tolerate the Methyl Parathion compounds, but not degrade it.

Tolerant of Paraoxon but not able to degrade it, and intolerable of methyl parathion. A gross colony count of around 615.

The sample was collected after a rainy day from in front of a sewer grate. The sample was consistency similar to clay and was somewhat wet at the time of collection.

<p>Sample collected near&nbsp;Stafford</p>

<p>Soil sample was collected from the northwest end of the bayou</p>

<p>&nbsp;</p>

<p>&nbsp;</p>

<p>Soil taken from 12 inches below frozen surface.&nbsp; Sample analyzed by Abigail Miller.</p>

<p>Sample collected at 12:04pm 8/25 and it was dizzling rain when collected</p>

<p>Week 0 Smells like death</p>

<p>Week 1 still smells pretty bad</p>

<p>Week 2 the smell starts to vanish</p>

<p>Week 3 smell and color vanishes</p>

<p>Week 4 no visible color or smell</p>

<p>Week 5 Paraoxon appears slightly yellow for Carbon and Sulfur Medias</p>

<p>Soil sample acquired from a ditch near a pipe on site of a water treatment plant in Slidell, Louisiana. 1 gram of sample is incubated&nbsp;in&nbsp;LB media for 1 week at 30 degrees celcius.</p>

Soil sample from front yard in a farm house (10 inches depth). the soil is very moisture and dark.

<p>South Campus - Collected next to an old cattle dipping chute</p>

Private alfalfa field in which Chlorpyrifos and lambda cyhalothrin were used during the 2017 growing season.

<p>The site is a alfalfa farm. It has a history of glyphosate and permethrin.</p>

<p>Private Alfalfa farm at which weed killer was the generally used spray during the 2019 growing season.&nbsp;</p>

<p>This sample was collected near a ditch with visible runoff from two nearby businesses: a concrete pipe company and a landscaping&nbsp;firm. The site was chosen based on its vicinity to two EPA Superfund sites. Within about 100 yards of the sample collection site, there was a children's playground and a middle school. I found the site by following an industrial truck carrying some sort of fluid. I watched that truck and two others like it back up to a manmade body water and dump their contents into the pool. My assumption was that this was normal behavior for the business and because of recent rains, the pool often overflowed into the area that I collected from.&nbsp;</p>

<p>The sample was collected at a creek side. The soil is moist, light brown in color, and clay-like texture. The creek shown sign of marine life.&nbsp;</p>

<p>The sample was founded near a Marine Training Center and a body of water connected to the Carpenter's Bayou, which is connected to Gulf Of Mexico. The area is also near&nbsp;various military buildings, a lot of ships because there is a port close by, and some manufacturing companies. There is a street drainage system that connects all the buildings.&nbsp;It did rain recently so the soil appeared mildly wet and dark.&nbsp;</p>

Institute alfalfa field where Karmex what used during the 2018 growing season.

<p>The sample degraded Paraoxon and used it for a carbon source and a sulfur source. It showed no signs of being able to degrade Methyl Parathion. It did seem slow to adapt to relying solely on the pesticide as a food souce, but once it adjusted it became every adept at it.</p>

<p>Soil collected from median lane between two driveways located near the building of MD Anderson Cancer Center Proton Therapy Center on the street of Old Spanish Trail, Houston, TX</p>

<p>The isolated bacteria degraded OP compound(s) on both MP &amp; Px Media</p>

<p>The sample had bacteria that were able to degrade organphosphorous hydrolase found in Paraoxon pesticide</p>

<p>Muddy earth</p>

<p>Paraoxon degraded</p>

<p>Sample was taken from behind a home garage because it was suspected to have been treated for ants with strong chemical insecticides.</p>

<p>CSM with paraoxon was yellow. Growth halo and tested positive. There was about 14 colonies on the plate</p>

<p>Sample was collected from field near North Main Street, Pearland TX. Although there was a positive result for paraoxon hydrolysis, there was no growth when added into the enriched media and incubated. There were a lack of microorganisms for the sample. Coordinates: 29° 35’ 30’’ N 95° 17’ 12’’ W</p>

http://maps.google.com/maps?q=Madden+Lane,+Houston,+TX&hl=en&ll=29.634464,-95.316696&spn=0.018166,0.030298&sll=29.636889,-95.316696&sspn=0.018166,0.030298&oq=madden+&hnear=Madden+Ln,+Houston,+Harris,+Texas+77048&t=m&z=15

<p>Sample was more like a mud texture, heavy rains over Memorial Day weekend. The sample was collected about 10 yards away from actual bayou water and about 8 inches below surface. It was moist, not too wet neither too dry. Its smell was very peculiar. Paraoxon (Px) Methyl Parathion (MP) CSM SSM CSM SSM WEEK 1 N/A N/A N/A N/A WEEK 2 slight yellow and turbid pronounce yellow and clear slight yellow and clear turbid white WEEK 3 slight yellow and turbid pronounce yellow and clear slight yellow and clear colorless and clear WEEK 4 slight yellow and turbid pronounce yellow and clear turbid white colorless and clear WEEK 5 slight yellow and turbid pronounce yellow and clear turbid white colorless and clear At time of screening for OP hydrolysis, there was no growth.</p>

<p>Muddy soil sample with unknown components.</p>

<p>Charles Milby park and Sims Bayou is down stream from Buffalo Bayou, where PCI Nitrogen - a fertilizer production facility. The sample bacteria were able to hydrolyze Paraoxon in the sulfur-selected medium after five weeks of dilution, transfer, and incubation.</p>

<p>Soil was collected from park close to University of Houston-Main Campus</p>

<p>The soil obtained was in my parent's garden.</p>

<p>The sample was taken in the backyard, near the trunk of a tree.</p>

<p>This sample was collected from a farm with freshly sewed soil from Rosenburg, Tx.</p>

<p>Soil sample obtain from a golf course nearby cinco ranch katy tx</p>

<p>The soil sample was collected from River Terrace Park on Lakeside Drive in Channelview, Texas.</p>

<p>Soil Collected from Sugarland Park NN</p>

Soil sample

Sample was taken from riverbed at Buffalo Bayou Park. Location was 15meters from a common running path. The site had indications that water levels were subject to constant change according to the weather. Soil sample was mostly sandlike in texture and fairly moist. Site was located under a bridge.

dirt spot: (29.7617512, -95.3861573)

<p>Soil sample collected near bank of Brays Bayou in Houston's 3rd Ward.</p>

<p>The presence of growth on my Glycerol Minimal Media agar plate indicates I have bacterial strains that are capable of tolerating the pesticide, Paraoxon. I also have a few colonies with halos which are an indication of hydrolysis, meaning that I also have a strain that might be capable of degrading OP compounds.&nbsp;</p>

<p>Sample collected approximately 10 inches below the top soil at around 12:35 P.M.&nbsp;</p>

<p>Collected soil sample. Weekly results.</p>

<p>&nbsp;</p>

<p>Farm run-off area</p>

This sample was taken on Jan. 9, 2020 from a private lawn that had recently been treated with Raid - Ant & Roach Killer.

<p>It was taken from a home garden used to grow tomatoes. There is one large tree to the east of the garden. The north side of the garden is exposed to the road</p>

<p>Location= Private home garden.&nbsp;</p>

<p>&nbsp;</p>

<p>Pesticide= Ortho Home Defense is used regularly.&nbsp;</p>

<p>The sample was collected at the edge of the Highland Acid Pits on Clear Lake Rd. The area is well known to be highly contaminated from years of dumping waste that has caused the water to become highly acidic. This is confirmed by my experience; the smell was strong. Wearing protective gear, I walked into the water and collected my sample from the depths of an eroded mound. There was a storm drain letting water into the pool that had visible rusting on the floor of the pool with red, metallic sediment all around it. There was no visible aquatic life in the pool, but there were several snakes surrounding the area. Next to the pool was a large generator, a mobile home community, and the Baytown Boat Club. I drove through a residential neighborhood to get to this location.&nbsp;</p>

<p>Taken from a flowerbed in the front lawn of my family home. Pesticides used during the active 2019 growing season were petroleum oil, Cyfluthrin, Imidacloprid, and Spiromesifen.</p>

Dirt sample near the University of Houston campus Fleming Building was taken. The sample was taken by digging about eight inches into the ground and taking about a 10 gram sample. The sample was used to isolate for OP degrading bacteria.

Colony 2 was a pure sample.

Gram-Status: Gram-negative

Oxidase Test: Negative

Catalase Test: Positive

Motility: Non-motile

Cell Shape and Size: rod-shaped and 0.5 um

<p>Halo was present surrounding Bacterial colonies in CSM- Px Agar plate indicating bacteria was able to hydrolyze organophosphorus compound and giving a positive result.</p>

<p>The soil bacteria sample was collected from the Japanese garden within Hermann park.</p>

<p>The soil sample was actually hard like clay and was very dark when I had collected it from the park</p>

<p>Muddy sample of soil</p>

<p>The soil sample was taken taken from a local park located in SugarLand. A 8-10 feet hole was dug into the ground and a mixture of wet and dry soil was collected in put into the soil collection tube. Over the course of 5 weeks it was noted that the Sulfur Selective Media with the Paraoxon was the best at showing pesticide degradation in bacteria. It was noted in week 4 and week 5 of the experiment that the the SSM+PX tube turned orange then, burnt orange. In the agar plate, it was noted that the PX resulted in two types of bacteria, the larger bacteria around the outside was able to hydrolyze and the bacteria towards the center was only able to tolerate the paraoxon.</p>

<p>this sample was collected from a bayou with the adress of 16600-17080 Beechnut st Houston TX 77083 on 09/08/2012. the sample was moist.</p>

<p>Kevin Smith sample 1</p>

Sample tested positive form paraoxon with about 26 white colonies. Halos are present which show sign of degradation.

<p>Soil sample collected from the bayou behind the apartment complex Bayou Oaks from University of Houston Housing. Sample collected from 12 inches deep ground for better chances of getting a better result.</p>

<p>Mound of dirt collected from about 3-6 inches deep near</p>

<p>Muddy soil sample</p>

<p>Sample was milk chocolate brown, with some moisture present.</p>

<p>The soil sample was dug from an area close to the big lake of the Kickerillo-Mischer Preserve in Houston. There was dead fish all over the margins of the lake because Harvey had brought fish from other areas (farms) that were not surviving to that particular environment.</p>

<p>The soil sample had a little moisture content when collected from the farm.</p>

<p>The sample was dry and clumpy dirt resembling nestle chocolate powder. It was collected from Theiss Farms. The sample tested negative for Organophosphorus degradation.</p>

<p>sample was collected about 10 yards from the receding water line, water of the bayou was about at 70% capacity during the time of collection because of a series of heavy rain falls from the weeks leading up to the collection which included a heavy flooding during memorial day weekend 2015. This meant that the location of collection was resting in standing water.</p>

<p>Sample was collected from a backyard garden. The area had 15 years of previous pesticide usage.</p>

<p>The soil sample was dark grey, sandy and smelled like gasoline/crude oil.</p>

<p>Sample was collected from the edge of a lake between a suburban and museum of natural sciences.</p>

Sample found near a neighborhood man made pond.

Before the temple was constructed, the area was flooded by Harvey Hurricane and the soil might contain potential bacteria that possess desirable strains.

<p>200 microliter inoculation of week 3's sample into fresh LB media. Samples have been shaken or one week in shaker machine (shook for four weeks total). SSM(MP) and CSM(MP) are clear, SSM(Px) is now clear after being slightly yellow, and CSM(Px) is slightly yellow.</p>

<p>Sample was obtained near the San Jacinto River, below US 59 North towards Cleveland.</p>

<p>was wet, clumped, and muddy.</p>

<p>Peach Orchard</p>

<p>Local plant nursery where&nbsp;Neem oil, Sulfur and Pyrethrin have been used recently.</p>

<p>Commercial Alfalfa Field in which Chlorpyrifos, Lambda-Cy, and Hexazinone was used in the 2019 growing season.</p>

<p>The site was the backyard of a home in a residential area. The area has multiple fruit trees. The owner had listed Roundup as a pesticide being used, but neither she nor myself were aware that that was an herbicide not a pesticide. The home was recently bought by the owner and could have had other pesticides used in the past, such as atrazine.&nbsp;</p>

<p>This sample was collected on the shoreline of a peninsula sticking into Crystal Bay that is directly adjacent to the oil refineries and industrial corridor of Houston. The location is a park with a large playground site, and while I was collecting, I noticed several people out fishing in the water. I chose this site because it was suggested by the lab instructor and there were previous soil samples collected here with positive results. I took the sample from an area close to the water where the soil was relatively soft from recent rains.&nbsp;</p>

<p>&nbsp;_ M3</p>

<p>Shelby Bannerts Soil Sample&nbsp; - I collected this soil sample on a ranch thats has farming activity occurring frequently. In the surrounding area was livestock and a creek bin. The ground was pretty damp when the sample was collected.&nbsp;</p>

<p>Soil sample from a home garden in Star, ID. Pesticides that have been used are multiple glyphosate-based pesticides.</p>

<p>Southdown Park - I chose this area because it is an area that pesticides are heavily used and is regularly serviced. The&nbsp;site was damp at the time of collection because of the rains the weekend prior. The site is at the edge of the park before a ditch and the northbound feter road off of Hwy 288.</p>

<p>a small soil sample was collected from the Mint beds in the botanical garden across from Miller Outdoor theater.</p>

<p>Approx. 10–20 grams of soil was collected at a depth of approx. 20–30 cm from a grass farm in Crosby, TX on January 24, 2016. The sample was stored in an opaque 50 mL tube and kept at room temperature (approx. 25ºC). The soil at the collection site was wet after a day of rain.</p>

<p>Soil was collected at a recreational park where kids play and people exercise. There is also a dog park.</p>

<p>Tolerant of Paraoxon. Intolerant of methyl parathion. 2 paraoxon hydrolyzing colonies.</p>

<p>Sample was taken from a small cotton farm that used pesticides. Bacteria was found to tolerate Paraoxon but not degrade it.</p>

<p>The sample is collected from cotton field. The sample was positive for Paraoxon and negative for Methyl parathion.</p>

<p>Soil Sample</p>

<p>Week 1 Px CSM-Yellow Px SSM-Slight Yellow Mp CSM-Yellow Mp SSM-Slight Yellow Week 2 Px CSM-Yellow Px SSM-Slight Yellow Mp CSM-White Mp SSM-Slight Yellow Week 3 Px CSM-Yellow Px SSM-Slight Yellow Mp CSM-White Mp SSM-Turbid White Week 4 Px CSM-Yellow Px SSM-Slight Yellow Mp CSM-White Mp SSM-Turbid White Week 5 Px CSM-Yellow Px SSM-Slight Yellow Mp CSM-White Mp SSM-Turbid White</p>

<p>Approximately 15 grams of moist Soil Sample was collected by the Miller Outdoor Theatre.</p>

<p>Soil Sample was taken from Blessington Farm. Originally thought to be laced with pesticides, but found out from the owners that minimal pesticides were used on their farms. Additionally, flood water submerged the farm back in April might had an impact on the bacteria community. The spot chosen on the farm was known to be with "most" pesticides, the soil collected was clay-like and smell like manures.</p>

<p>Muddy soil sample</p>

<p>Many small colonies. No hydrolysis is presented on the sample. No Methyl Parathion growth was presented on the plate.</p>

<p>Soil collected from hole approximately 10 inches deep three days after flood waters receded.</p>

<p>Moist and dense soil extracted from Bayland Park Dr near a baseball field from an ~8 inch deep hole in the ground.</p>

<p>My sample contains approximately 80-95 positive tolerant colonies. The tolerant bacterial colonies weakly or incompletely degraded CSM media containing Paraoxon pesticide to grow and survive.</p>

<p>Grayish Brown and dry soil sample. It was collected from the land where it was farmland before.</p>

<p>front yard of a house in farming area in Jersey Village (Houston, Texas). 8 inches deep. The sample looks dark and moisture when i got it fromt he yard.</p>

<p>Soil collected from a home garden and pesticides are used consistently in this garden. 10g of Soil sample was collected into 50mL amber tube</p>

<p>Soil was just a brown color, with a bit of a red tint. Did not really smell out of the ordinary, just smelled like regular dirt.</p>

<p>Found near a residential man-made pond.</p>

The sample was collected by the Brays Bayou near the University of Houston.

The sample was collected from an area where the water had contact with the soil. The water has heightened levels of pollution due to the amount of ships that use this waterway daily and the amount of industries that have likely used this area as a dumping ground for their waste material.

<p>Found soil along a stream in Crighton Park in Conroe, Texas.&nbsp;</p>

<p>200 microliter inoculation of week 4's soil sample into fresh LB media. Samples have been shook for one week in shaker machine (shook for 5 weeks total). SSM(MP) and CSM(MP) are clear, SSM(Px) is a very faint yellow, and CSM(Px) is yellow.</p>

<p>This soil sample was taken from beside the road in front of two chemical factories, located in/near Almeda Heights close to Pearland, besides Beltway 8. Could not get into the small street right in-between the two factories as it was gated.</p>

<p>Cow Pasture</p>

<p>Private alfalfa field at which mustang maxx was used during the 2019 growing season.</p>

<p>Alfalfa Field at which Velpar was last used during the 2019 growing season.</p>

<p>Private home garden which used neonicotinoids during 2019.</p>

<p>I chose this location based on its vicinity to Buffalo Bayou and the EPA Superfund Site Patrick Bayou. The location is a park near a commemorating memorial to the Republic of Texas called the San Jacinto Monument. This spot sits at the mouth of the Buffalo Bayou and Houston Ship Channel where hundreds of ships travel in and out every day. There is a ferry at the end of the land mass along with a restaurant. There were also several warning signs posted near the water's edge banning visitors from fishing or swimming in the water. To get the sample, I climbed down to the edge of the shoreline and dug my hole where the grass was growing. There was litter and visible oil marks on the shore that I could see.&nbsp;</p>

The site is a small home garden, used as recently as the growing season of 2019, where cucumbers and tomatoes are grown. The pesticide used in this garden was Amdro Ant Block.

<p>Soil sample collected near the Buffalo Bayou at Addicks &amp; Barker reservoirs&nbsp;in a flat, grassland-like area.</p>

<p>Rural landscaping, soil and rock cover where alligar 2 and 4-D Amine have been used over the last 10 years.</p>

<p>Sample was collected from a garden that is regularly treated with pesticide. However, there was no growth on the plates.</p>

<p>I collected my sample from a corn maze, the ground was wet due to raining. I dug ground about 6-7 inches and collected sample. I stored sample in a dark tube and kept it in mild environment.</p>

<p>Tommy Lo Week 1 Paraoxon Methyl Parathion CSM PSM SSM CSM PSM SSM N N N N N N Week 2 W W W W W W Week 3 T T T T T T Week 4 T T S T T T Week 5 S S C W T T</p>

<p>Sample taken from Memorial Park Golf Course located at 6051 Memorial Dr. Houston, TX 77007. U.S.</p>

<p>The Soil Sample was collected in Channel View river bank by a public park around 5:27pm at 8/26/2016. The longitude and latitude was 29°46’57”N 96°6’9”W. The soil consistency was dark grey and very clay-like.</p>

<p>There was growth on the plate and few halos, so it is deduced that the bacteria is able to tolerate the pesticide and not necessarily degrade it. This could be evidence that a degrading bacteria was missed along the process.</p>

<p>Sample was collected from a small plot of soil used communicably by the apartment complex residents.</p>

<p>Soil sample was collected at 9:10 AM on August 27, 2010</p>

<p>Week 1 Paraoxon Methyl Parathion Student CSM PSM SSM CSM PSM SSM Kim N. Y C N N N N Week 2 Paraoxon Methyl Parathion Student CSM PSM SSM CSM PSM SSM Kim N. W W W W W W Week 3 Paraoxon Methyl Parathion Student CSM PSM SSM CSM PSM SSM Kim N. S T T S T T Week 4 Paraoxon Methyl Parathion Student CSM PSM SSM CSM PSM SSM Kim N. S T W S T T Week 5 Paraoxon Methyl Parathion Student CSM PSM SSM CSM PSM SSM Kim N. S T T S T S</p>

<p>Date: September 21, 2011 Week 1 Paraoxon (Px)+ CSM = So Paraoxon (Px)+ SSM = So Methyl Parathion (MP) + CSM = So Methyl Parathion (MP) + SSM = W Date: September 28, 2011 WEEK 2 Paraoxon (Px)+ CSM = Y Paraoxon (Px)+ SSM = S Methyl Parathion (MP) + CSM = S Methyl Parathion (MP) + SSM = W Date: October 05, 2011 WEEK 3 Paraoxon (Px)+ CSM = C Paraoxon (Px)+ SSM = Y Methyl Parathion (MP) + CSM = W Methyl Parathion (MP) + SSM = S Date: October 12, 2011 WEEK 4 Paraoxon (Px)+ CSM = T Paraoxon (Px)+ SSM = Y Methyl Parathion (MP) + CSM = W Methyl Parathion (MP) + SSM = C Date: October 19, 2011 WEEK 5 Paraoxon (Px)+ CSM = Y Paraoxon (Px)+ SSM = Y Methyl Parathion (MP) + CSM = W Methyl Parathion (MP) + SSM = C Legend So- same color as soil inoculate W- turbid white T- colorless Y- Pronounced yellow color S- Slight yellow color C- Yellow color and lightly turbid</p>

<p>Soil sample from front yard in a farm house (10 inches depth). the soil is very moisture and dark.</p>

<p>Sample taken from a park that had water from the Old River, that connects to the San Jacinto River.</p>

<p>Soil sample was collected from a cotton farmland located in the Rosenberg area of Texas.</p>

<p>This is a community farm where pesticide is not used. Approximately 100 bacterial colonies growth seen on agar plate with Paraoxon. Half of them appeared white with a halo ring in the surrounding and the other half appeared white. There is indication of bacteria that tolerate Paraoxon and bacteria that degrade paraoxon in the soil sample.</p>

<p>Sample Ricefield - was wet, clumped, and muddy.</p>

<p>Soil sample was collected on the banks of the Buffalo Bayou. There were men fishing and a great deal of trash (beer cans, used condoms, etc) in the area.</p>

<p>Soil collected from a small runoff from Brays Bayou located on Chimney Rock near South Braeswood.</p>

I had colony growth with a few cells that had Halos, indicative of OP degradation.

Sample was collected from a creek bed at a residential park.

<p>week 0 soil sample from george bush park</p>

<p>Inoculation of CSM Px tube was plated on minimal media agar plate. The CSM Px tube had the most prevalent yellow coloration, indicating it may be able to degrade organophosphorus compounds; This is why CSM Px was chosen to be plated.</p>

<p>The sample was collected&nbsp; in the Stude Park surrounded&nbsp;by&nbsp;a lot of plants.&nbsp;</p>

<p>This sample was collected near the Brazos River. The bacteria isolated was found to tolerate Paraoxon but unable to hydrolyze it.</p>

<p>Soil sample collected from Ballester's Fishing Area in Morgan's Point, Texas. Results are negative for OP hyldrolysis.&nbsp;</p>

<p>Cow pasture</p>

Alfalfa field, Lorsban was used in the 2019 and 2018 growing season.

<p>Public corn field at which atrazine has been&nbsp;used in the past. It has been such a long time since use, that farmer did not remember specific time.</p>

<p>Soil collected from Sky Mountain Golf Course where Bifenthrin was used in the 2019 spring.&nbsp;</p>

<p>Collected from backyard of residence on January 8 2020 at 430pm. Unknown if any pesticides have been used.</p>

<p>Had recently rained the night before, very muddy, clay-like&nbsp;texture. Paraoxon positive with 25 colonies and 13 halos. Methyl Parathion negative.</p>

<p>Alfalfa&nbsp;Hay Pasture. No mention of pesticides used.</p>

<p>My sample was very moist prior to inoculation. My bacteria was plated on CSM agar with Paraoxon and developed no significant growth.</p>

<p>Dirt was collect at a cotton farm by hwy 99 at the corner of morton rd and harlem rd in richmond tx at 7:30pm</p>

<p>The area where the sample was obtained is known to have their course exposed to pesticides in order to keep the grass healthy. However no results where obtained at all. This could have to do with the that part of the golf course might be new or the course isn't as exposed to pesticides as one would think.</p>

<p>This soil sample was collected at Four Seasons Park near Katy area. The time was around 5:00pm on August 30, 2016. The latitude is N 29°50'25.956'' and the longitude is W 95°48'2406''.</p>

<p>Bacteria tolerant to PX. Several colonies showed halos around the edges which means there is a presence of hydrolysis of PX.</p>

<p>(Student: Alyssa Nguyen) Soil sample taken near bank of Buffalo Bayou after Hurricane Harvey - Dug about 5 inches. Had a lot of clay when obtained.</p>

<p>one of the agar plates has too many colonies therefore it was difficult to count but the second agar plate has many small colonies. the fuzzyiness around the colonies means degrading.</p>

<p>Farm Soil in Rosharon, TX</p>

<p>Px CSM Weeks 1-5: CYYYY Px SSM Weeks 1-5: WWTTT MP CSM Weeks 1-5: SSSSS MP SSM Weeks 1-5: WWTTT Plated on CSM agar with PX and MP. Growth with halo in both PX and MP. Preliminary Hydrolysis Test was Positive in both PX and MP. Location on Farm to Market Road 521 (approximate address 11730) in between and parallel to Willow Lake and County Road 55. Coordinates: 29.413507,-95.477886</p>

<p>Sample was collected from an onion patch at Froberg's Farm in Alvin, Texas.</p>

<p>The cafe has a garden behind the shop. This sample is collected from that location</p>

<p>The soil sample was collected from Texas Avenue Park located in Webster, Texas. The sample was put into tubes containing media with two different organophosphorus compounds, Methyl Parathion and Paraoxon. The microorganisms were able to degrade Paraoxon, but not Methyl Parathion.</p>

<p>A large cell clump covering in the plate. Some hydrolysis is presented on the sample. No Methyl Paraxon growth was presented on the plate.</p>

<p>The sample was taken from Buffalo Bayou Park after heavy rainfall.</p>

<p>The location from where the sample was taken from had previous exposure to pesticides, and was taken from the backyard of a garden in the Northwest Houston Area.</p>

<p>Sample collected from 1620 N Houston Ave, Humble, TX 77338. Some colonies were very large in diameter compared to other students' results. The ring of hydrolysis is very small.</p>

<p>The soil sample was collected from a vegetable garden in my backyard. The soil sample was dry and crumbly. The results were negative for degrading bacteria.</p>

<p>Soil collect from Shadow Hawk Golf Club</p>

<p>Sample was obtained at a home garden it showed Paraoxon tolerance.</p>

<p>This sample was collected in a public park by a bank of along the San Jacinto River</p>

<p>moist soil off the banks of Willow Waterhole Bayou offshoot, clumpy and clay-like</p>

<p>The location the soil sample was taken from a vegetable farm at 3217 Spring Cypress Rd, Cypress TX 77388.<br />
An 8-12 inch deep was dug at this location and about 10g sample was taken. It was stored in a container that protected the sample from UV sunlight. In addition the surrounding temperature was kept at room temperature.</p>

I had a colony overgrowth.

Sample collected from: Northwest Houston from the soil near the outskirts of a retention pond.
Coordinates 29.988894, -95.475892
Colonies were tolerant when grown in PX GMM Plate, but showed no sign of OP degradation.

the sample was collected on the bank of the San Jacinto near S.Burnet Dr. It was collected on the back yard of an abandoned house where there was access to the shore.

<p>PX MP negative due to only having growth but not hollows around it. sample cannot break down OP</p>

<p>Week 0 soil</p>

<p>light yellow turbid solution with dirt visible at the bottom and top of the sample</p>

<p>yard</p>

Home garden where glyphosate and some members of the neonicotinoid family were used during the summer of 2018.

<p>Grass pasture used for horses at the SUU farm.</p>

<p>The sample was taken from a private cornfield in which Quin Clorac was used in the 2016 season.</p>

<p>The soil sample was taken along the park's east jogging trail. I followed the trail until I came to&nbsp;a fenced off junkyard. There was a path off the trail, adjacent to the fence, allowing me to take a short hike down until the path ended. I was only a few feet away from the waters of Buffalo Bayou. The soil was a light brown color and extremely damp as it had been raining all day.&nbsp;</p>

<p>Personal rose bed. Pesticides applied in 2019 growth season is triazicide by spectracide.</p>

<p>Alfalfa Hay Pasture. No known pesticides reported.</p>

<p>The soil sample was black, and it was collected from a cotton field on September 1st, 2014. It tested positive for paraoxon and negative for methyl parathion. After ten days of incubation, it was observed too many to count (TMTC) colonies on the minimal agar plates. However, I counted approximately 22,069 colonies.</p>

<p>This sample was taken from a garden in a residential area near community tennis courts in Misty Meadow Ln, Houston, TX.</p>

<p>It was obtained from a muddy region by the technology annex building</p>

<p>Collected in Sugar Land, TX. Degradation of Bacteria on OP Compound Paraoxon</p>

<p>Old Richmond Rd and Copano Bay Drive. It came from a little flower patch.</p>

<p>Sample taken from a crop field currently in use in Alvin, TX.</p>

<p>The soil sample came out to be positive for paraoxon and was able to withstand the pesticide.</p>

<p>Digging a hole 12 inches into the ground and obtaining the soil in a sample container tube.</p>

<p>A hole was was dug approximately 12 inches deep, right off the trail, about 8 feet from the bayou water, and approximately 20-gram soil sample was collected, then placed in a 50mL amber tube. The soil sample was a mixture of moist and dried dirt.</p>

<p>Soil collection from the Brazos river near a recreational park on a rainy day. Sample tolerated well in minimal medium but demonstrated no sight of hydrolysis.</p>

<p>Sample was collected from my home garden, which is treated with pesticides every 3 months. Regardless, no evidence of pesticide degrading or tolerant bacteria was found.</p>

<p>Soil was extracted from ~10" under ground.</p>

<p>The sample was mostly dry, but some parts were wet. The sample showed no sign of hydrolysis therefore was not inoculated into rich media and was not plated onto a plate</p>

<p>The soil sample was collected on August 27, 2016 from Atkinson Farms. The plated sample displayed positive growth after exposure to Paraoxon and negative growth after exposure to Methyl Parathion.</p>

<p>The soil sample was taken from dirt that was slightly wet due to rain the previous day. The soil's location is near a river source, but also near a residential area.</p>

<p>Sample was top layer of clay and middle layer of sand. The sample was also very muddy.</p>

<p>Soil sample was collected from a citrus tree in a residential backyard.&nbsp;</p>

Located by the fountain

The soil was collected at a water site near Greens Bayou.

<p>Sample was collected at Sugar Land Memorial Park.</p>

<p>Strain could tolerate pesticide but not degrade it. Negative Result.</p>

<p>Average Colony count= 2,016</p>

<p>Soil was collected, and submitted to professor from Sugarland Memorial&nbsp;Park.</p>

<p>Verdant Tree Farm Soil Collection</p>

<p dir="ltr">This sample was collected by the Buffalo Bayou&nbsp;at Buffalo Bayou Park.</p>

<p><strong>Enterobacter aerogenes PX01&nbsp;</strong></p>

<p>Hideaway Park</p>

<p>Clay like soil on the bank of the buffalo bayou, near the kirkwood overpass, lots of trash.</p>

<p>This sample was acquired from the parking lot of a plant nursery. No known pesticides have been used in this location, but herbicides such as glyphosate were used.&nbsp;</p>

Small garden in an area of dirt by a sidewalk. Pesticide uses were unknown for this location.

<p>Soil Sample From George Bush Park - On the day of&nbsp; the soil retrieval, the soil was damp and the environment was moist from the morning dew. The soil had a brownish color and had a soft and malleable texture. The area where I collected my soil sample is where Buffalo Bayou intersects and meets with an artificial river flowing from a nearby neighborhood and near George Bush Park Boardwalk.&nbsp;</p>

<p>The soil collected&nbsp;clumped and damp. It was yellow brown in color with a clay-like texture. It was collected near a water treatment facility.&nbsp;</p>

<p>&nbsp; converted farm to residential area</p>

<p>Collected from the backyard of my home.</p>

Soil Sample was collected from cotton field on Benton Rd in Richmond, Texas

<p>A soil sample collected from a grass farm in Crosby Texas.</p>

<p>The soil sample presented OP degradation</p>

<p>After 5 weeks of testing by progressively diluting the bacteria sample in SSM (Sulfur selective media) and CSM (Carbon Selective media) in both Methyl Parathion and Paraoxon condition it was determined what type organophosphatase activity is found in this bacteria sample after growth in a media rich dish. SSM consistently had more turbidity than the CSM (Carbon selective media) , but only the PX (paraoxon) samples ever had a breakdown to form the yellow color p-nitrophenol product in both, turbidity or no; MP, or Methyl Parathion media NEVER had yellow coloration. This trend stayed throughout, but eventually only the CSM had a yellow color in the 5th week trial with almost no turbidity in any sample. The final trial's SSM PX should still have had a yellow color, but it may have been due to a too great dilution of the soil bacteria by this point that it could not break down the Paraoxon, whereas the CSM-PX had just enough to break down the Paraoxon. However, my bacteria in particular grew best on an SSM based on turbidity consistently staying higher in the SSM tubes. This means that it is a bacteria that breaks down Paraoxon in order to obtain its sulfur or carbon products; the slightly higher level of turbidity in the SSM media throughout the 5 weeks may indicate the bacteria prefers sulfur for growth.</p>

<p>CSM with Paraoxion was yellow in color, had some growth when plated on CSM agar, tested positive</p>

<p>Sample taken from rural area in South Houston on FM 2234. Exhibited a positive reaction with Px + SSM. Showed evidence of OP hydrolysis.</p>

<p>This sample was collected from the nearby shore of Rio Grande, the main river that divides the US with Mexico along Texas. It is noted that a significant amount of contaminants run down this river, making it a very suitable place for data collection. The sample was taken from the US side in a nearby zone close to the international Bridge Juarez Lincoln. During the incubation and transferring process of 5 weeks, the sample displayed increased activity in Paraoxon CSM enriched media with a noticeable yellow coloration, whereas the rest of the tubes did not show any activity and progressively faded into a complete clear coloration. After plating and growth the whole plate was completely covered and made colony count not possible, however prominent white/yellow colonies can be seen which are approximately 200. These colonies are possible zones of hydrolysis of bacteria that is expressing an OPH gene.</p>

<p>An 12 inches hole was dig and soil was obtain. The soil sample was first inoculated in an enriched media and incubate at 37 degree Celsius. The soil containing media was then pipettes in minimal medical containing paraoxon and methyl parathion. Again was incubate for 7 day at 37 degree Celsius. This process was repeated until week 5. Then plated onto minimal agar with pesticide to observe for OP hydrolysis.</p>

<p>Bacteria collected and cultivated.</p>

<p>Retrieved near Brazos River</p>

<p>the sample was collected near the river.</p>

<p>Brown clumpy soil. no scent. Citrus fruit. 1 foot beneath the surface.</p>

<p>Sample taken from garden with hopes of finding organophosphorous degrading bacteria</p>

<p>This site is near Greens Bayou, which was contaminated with DDT. There was a concrete slab inside plastic fabrication plant, upstream, that had stains from liquid dripping into the ditch. Likely from rain, but may also have carried contaminants from the plant into the ditch. Sample was taken from this ditch close to the drain by a back gate, see attached image. Sample was taken halfway down; despite being above the water table below sample was mostly mud.</p>

<p>Soil collected from a home garden.</p>

<p>The soil sample was collected from the side of Berry Bayou. A hole was dug, 6-8 inches deep, and the soil at the bottom of the hole was collected and stored into a dark tube.The sample was kept at room temperature.</p>

<p>Soil sample collected from a bank on the San Jacinto River Bay near the Fred Hartman Bridge. Spring 2018</p>

<p>Sample was collected from an onion patch at Froberg's Farm in Alvin, Texas.&nbsp;</p>

Yellow coloration around some bacterial colonies indicates some bacterial hydrolysis of the pesticide paraoxon.

<p>This soil sample was taken from Sugar Land Memorial Park near the Brazos River in Texas. Negative result Gross cell count: approx. 2016 cells</p>

<p>The soil sample was collected from a retention basin that flooded during Hurricane Harvey.&nbsp;</p>

<p>The soil sample was collected from the banks of the Buffalo Bayou Hike &amp; Bike Trail near the University of Houston-Downtown.</p>

<p>Soil was collected at Buffalo Bayou Park, all data and results were recorded and documented.</p>

<p><strong>Raoultella ornithinolytica PX02</strong>&nbsp;</p>

<p>Greenhouse</p>

<p>A private home above ground box garden for squash. Was treated with Sevin (Carbaryl) in the 2019 growing season.&nbsp;</p>

<p>Home lawn, located in Spanish Fork, UT. Sprayed with TruGreen signature blend, during the summer of 2016.&nbsp;</p>

Soil collected from the perimeter of an apartment building where bifenthrin and cypermethrin pesticides were used during the summer of 2019.

<p>"Star Nursery", a plant nursery/garden store</p>

<p>The exact pesticides are unknown due to issues contacting the plant vendor, but the staff's best estimate was neem oil.</p>

<p>Backyard where sevin was used during 2018.</p>

<p>Sample Collected on January 25 at 1426. The location was in River Terrace Park in Channelview Texas, 77530. Along the rocks&nbsp;wall between the Old River and land. In the immediate vicinity, there is a playground and a community of homes directly across the street.&nbsp;&nbsp;</p>

<p>My soil sample was collected from my backyard. We occasionally treat for insects. The bacteria in the sample was paraoxon positive and methyl parathion negative.</p>

<p>No significant color changes were found after 5 weeks of inoculation in Methyl Parathion and Paraoxon.Sample was taken 5 meters into cotton field immediately northwest of the Student Parking Lot.</p>

<p>Soiled sample collected from a field used to grow vegetables on Stuebner Airline Rd.</p>

<p>Soil collected from the banks of McGovern Lake outside of the Houston Zoo.</p>

<p>Sample taken from bank of San Jacinto river near market street.</p>

<p>Soil collected from a former cotton farm in Rosenberg, TX.</p>

<p>Collected in or near an area that was cultivated for rice 50 years ago.</p>

<p>An unofficial count of the colonies yields an estimation of about 79 total colonies, with 51 OP degrading colonies, and 28 OP tolerant colonies.</p>

<p>Grew well when inoculated with CSM Paraoxon. Plated on CSM agar with Paraoxon. Plating results showed growth with halo. Bacteria sample tested positive for Preliminary Hydrolysis Test.</p>

<p>Soil was collected from Mason Park near a marsh. After liquid media culturing and screening on a minimal agar plate, the sample was found to be Paraoxon Positive and Methyl Parathion Negative.</p>

<p>I got my sample from a bayou by the Fort Bend County fairground in Rosenberg.</p>

<p>The sample was mostly solid with a little bit of twigs and sand mixed in.</p>

<p>This soil sample was taken from within the Barker Reservoir after Hurricane Harvey.</p>

<p>My sample appears to be from farm land. My starting sample was not as soft as other student's samples. The soil I collected had a texture more like clay than regular soil.</p>

<p>The collected soil was from Froberg Farm, where several vegetables along with strawberries are grown. The soil was dark brown in color and heavily moistened. During the process of isolating and growing the bacteria present in the soil sample, I have observed color changing patterns in a weekly basis on specific combination of medium and insecticide. The results are as follows: Week 1, 9/21/2011 Paraoxon (Px)+ CSM = pronounce yellow color (Y) Paraoxon (Px)+ SSM = yellow color and lightly turbid, (C) Methyl Parathion (MP) + CSM = pronounce yellow color (Y) Methyl Parathion (MP) + SSM = yellow color and lightly turbid, (C) Week 2, 9/28/2011 Paraoxon (Px)+ CSM = pronounce yellow color (Y) Paraoxon (Px)+ SSM = Colorless (T) Methyl Parathion (MP) + CSM = yellow color and lightly turbid (C) Methyl Parathion (MP) + SSM = Colorless (T) Week 3, 10/05/2011 Paraoxon (Px)+ CSM = pronounce yellow color (Y) Paraoxon (Px)+ SSM = Colorless (T) Methyl Parathion (MP) + CSM = yellow color and lightly turbid (C) Methyl Parathion (MP) + SSM = Colorless (T) Week 4, 10/12/2011 Paraoxon (Px)+ CSM = pronounce yellow color (Y) Paraoxon (Px)+ SSM = Colorless (T) Methyl Parathion (MP) + CSM = yellow color and lightly turbid (C) Methyl Parathion (MP) + SSM = Colorless (T) Week 5, 10/19/2011 Paraoxon (Px)+ CSM = pronounce yellow color (Y) Paraoxon (Px)+ SSM = Colorless (T) Methyl Parathion (MP) + CSM = yellow color and lightly turbid (C) Methyl Parathion (MP) + SSM = Colorless (T) Around 80-90 colonies of bacteria were successfully isolated from the soil sample and grown well on the CSM Agar media with Paraoxon pesticide</p>

<p>Soil sample was collected in a residential backyard. It was sampled from a citrus tree.</p>

<p>Sample collected at the surface, dry and the land was not used to grow anything. Did not expect to find pesticide degrading bacteria.</p>

<p>Soil Sample: Sandy soil slightly moist from the bank of the bayou located inside of the park with debris spread within.</p>

<p>soil collection from Bayou Parkland near Brays Bayou</p>

<p>It was muddy, and wet.</p>

<p>3 feet from the river</p>

Sample of soil was collected from cemetery

<p>This sample was collected from Buffalo Bayou, the soil is used in Module I for Biotechnology research methods lab.</p>

<p>Soil sample will be tested to see whether any bacteria can biodegrade pesticides.</p>

<p>Soil taken from 12 inches below the frozen surface.&nbsp; Analyzed by Abigail Miller and Kara Morrison.</p>

Pseudomonas nitroreducens DF05

<p>Wastewater treatment facility</p>

<p>A private home garden and lawn where Triazicide has been used for the last several years at the beginning of the growing season.&nbsp;</p>

<p>This is a private garden that used the pesticide Sevin (active ingredient: Carbaryl) was used in the 2019 growing season.&nbsp;</p>

<p>Family garden at which Sevins Powder, which contains carbaryl, has been used during the 2019 growing season.</p>

<p>The sample was collected in an isolated location not too far from a park. The area from which the sample was collected was also near a bayou. There were many trees and untended patches of land. The sample itself had a very rough consistency and was light brown. It was slightly moist, possibly because of the rain from the day before. The sample was collected by digging nearly 8 inches near the root area of a tree.&nbsp;</p>

This sample was collected from a private hay field that has been treated with malathion in the past. The ground was frozen when the sample was collected.

<p>This was collected from my home yard underneath a pomegranate tree.</p>

<p>Cyper tc is the only recent pesticide use, it has been used for over 2 years</p>

<p>Very small colonies, non-clustered, white in color, numbering around 80 total. No immediate signs of clearing or degradation to indicate Paraoxon hydrolysis; however, there is sufficient evidence of growth.</p>

<p>i have collected a soil sample from a flowerbed located in the Gulfgate (620 and I45s)</p>

<p>The soil sample was collected from a residential ranch in Dayton, Texas that used to have an area for vegetation growth.</p>

<p>The bacteria collected were inoculated in both Methyl Parathion and Paraoxon for 5 weeks after allowing it to grow in a LB broth for a week. The Methyl Parathion samples showed no signs of life by week 3, but the sample was able to grow and digest Paraoxon. Turbidity was observed in all of the sample tubes for the first 2 weeks.</p>

<p>Sample was collected to test for hydrolysis of op compound</p>

<p>First Paraoxon Plating 88 was at a 1:100 200ul sample Second Paraoxon Plating 16 was at a 1:1000 200ul sample</p>

<p>SAMPLE WAS WET AND THE FARM WAS IN OPERATION. THEY GREW FRUITS AND VEGETABLES AND SOLD TO CUSTOMERS.</p>

<p>The sample was acquired from a private church garden in Northwest Houston from an area that had recently been planted with corn and other harvest crops nearby. The sample was positive for Paraoxon tolerance and was able to degrade (hydrolyze) the pesticide and OP compounds.</p>

<p>Clay like texture to the soil and it was wet</p>

<p>Soil sample of about 10-20 grams found behind house, in a bayou that gets flooded occasionally.</p>

<p>sample collected along sidewalk of Sabine Park, after Hurricane Harvey Flood.</p>

<p>Sample was collected by the banks of the buffalo bayou. Because of the recent hurricane Harvey, the bayou was overflowed and some soil might have been washed out.</p>

<p>The sample was collected from a strawberry farm in Fresno, TX. The sample tested positive for Paraxon and yielded tolerant colonies.</p>

<p>sample tested positive for CSM-Px pesticide.</p>

<p>Soil collected from JRN Nursery.</p>

<p>Soil was collected in a backyard in a suburb in Killeen, Texas. The soil was collected close to the house as the house gets regularly sprayed for bugs.</p>

<p>PX and MP screening for OP hydrolysis</p>

<p>Mud</p>

<p>Sample was collected from the banks of Brays Bayou next to the Brays Bayou Greenway Trial. The sample was dug up from about 6-8 Inches deep. The sample was collected after a flood therefore the soil sample was very damp and had the texture of clay.</p>

Sample was taken along the ditch line, about 8 - 12 inches deep

Located near the edge of Brays Bayou, sample was collected around 8:35 on the morning of the 10th of September, 2018.

200 microliter inoculation of Week 1's samples into fresh LB media; samples have been shaken for 1 week in shaker machine (shook for 2 weeks total). SSM(MP) and CSM(MP) are clear, SSM(Px) is slightly yellow, and CSM(Px) is yellow.

<p>Moist brown soil taken around 7 inches deep.</p>

<p>Location where soil sample was collected and the first tube inoculated with soil sample in LB media.</p>

<p>Soil sample taken from 12 inches below the frozen surface.&nbsp; Sample analyzed by Zachary Ward.</p>

<p>Sample collected near contaminated soil in Houston Tx and grown in two different media SSM and CSM, ass well as in two different types of pesticide per media.</p>

<p><strong>Stenotrophomonas maltophilia DF07&nbsp;</strong></p>

<p>backyard</p>

<p>Commercial pumpkin patch in which farafas and lambisai was used for the 2019 growing season.</p>

<p>Backyard plant bed in which Demand CS was used.</p>

<p>Home garden, under a pomegranate&nbsp;tree where cypermethrin has been used for at least two years.</p>

<p>Keegans Bayou Soil Sample - Soil Sample collected by Keegans Bayou, about 6 yards from the water line at 3:51 PM, 02/02/20.&nbsp;</p>

<p>&nbsp;Brays Bayou - Dark, moist soil from the bank of a nearby pond of where Brays Bayou ends on the west side.</p>

<p>Sample origen: La Porte Tx, 77571</p>

<p>Sample was taken from a backyard in a residential home in Houston. The sample was positive for Paraoxon tolerance and was able to degrade the pesticide and OP compounds.</p>

<p>Sample displayed tolerance for Methyl parathion on CSM.</p>

<p>It was collected in farm on the country side of Bandera county, San Antonio, Texas. The soil sample was collected from a organic farm where they grow tomatoes, chilies, etc. The soil was dry and hard to found a moist collection. The hole were the soil was collected was about 8cm deep. Sample was taken to the lab 4 days after collection. It was kept in room temperature all the time.</p>

<p>All clay</p>

<p>Soil sample was collected on the banks of the Buffalo Bayou. There were men fishing and a great deal of trash (beer cans, used condoms, etc) in the area. Dug about 10 inches down and collected sample in an amber tube and kept at room temperature.</p>

<p>The soil sample was collected on September 5, 2012. It was continuously stored at room temperature.</p>

<p>Soil sample taken from Froberg's Farms.</p>

<p>Sample was taken from a residential that have been treated for ants with strong chemical insecticides.The agar plates has a large number of very small colonies.</p>

<p>A sample of soil was collected from Cimarron Elementary. It was tested for OP degrading bacteria and produced negative results.</p>

<p>Sample taken from landscaped garden plot near fountain in Buffalo Bayou Park.</p>

<p>Sample taken from the edge of the White Lake in Cullinan Park.</p>

<p>The soil sample was collected from a park. The sample was tested postivite for Paraxon and bacteria tolerated the toxic environmnet.</p>

<p>The MP samples did not show strong evidence of hydrolysis at all. MP plates did not show any growth 29°54'49.1"N 95°42'13.3"W</p>

<p>Soil was collected from a flower bed in Cypress, TX 77433</p>

<p>The bacteria sample collected was able to degrade the PX pesticide, but was unable to degrade the MP pesticide</p>

<p>Soil Sample from Mary Jo Peckham Park in Katy, Texas.</p>

<p>Found near a gulf course by the Steep Bank Creek</p>

My sample was collected on August 26th, in League City, TX 77573.
Coordinates:
29.548035, -95.063081

1,256 colony count.
Positive results.
Sample is able to degrade to OP compound known as Px.

1g of soil in 6mL of LB media after being shaken in a shaker machine for one week. SSM(MP) and SSM(Px) are cloudy, CSM(MP) is slightly yellow, and CSM(Px) is yellow.

<p>Sample taken from 12 inches below the frozen surface.&nbsp; Soil analyzed by Ben Hall and Breanna Laber.</p>

<p>Soil sample placed into enriched media partially sealed to allow the bacteria that is found within the medium to breathe, and incubated for 1 week at a temperature of 37 degrees Celcius inside the rotational incubator to simulate a controlled environment&nbsp;</p>

<p>Sample results displayed are a week after the initial incubation process</p>

<p>Px CSM Weeks 1-5: CYYYY Px SSM Weeks 1-5: WWTTT MP CSM Weeks 1-5: SSSSS MP SSM Weeks 1-5: WWTTT Plated on CSM agar with PX and MP. Growth with halo in both PX and MP. Preliminary Hydrolysis Test was Positive in both PX and MP. Location on Farm to Market Road 521 (approximate address 11730) in between and parallel to Willow Lake and County Road 55. Coordinates: 29.413507,-95.477886</p>

Microbacterium sp. AISO3 

<p>Near Katy Hockley Rd. and West Rd. near the cricket field, at Paul D Rushing Park. Source of soil was in about 20 feet proximity to the body of water there. The soil was quite dense, and wet. Although it was dark, it had a slight brown hue to it. The soil collected was about 10 inches deep into the ground.&nbsp;</p>

<p>This sample was taken outside of&nbsp;the TDCJ Darrington Unit.&nbsp;There was plowed farmland on both sides of the road and all around this general location but nothing growing at the time this sample was collected. The sample was taken from a hole I dug next to the plowed farmland where grass was growing.&nbsp;The ground was soft and muddy. Upon digging it was noticed that the ground had&nbsp;many little rocks and some grassroots present. The soil was dark brown and I did not observe any bugs in the 12'' hole I dug, no water pooled at the bottom of the hole.&nbsp;</p>

<p>Home garden growing fruits and vegetables in which the termiticide&nbsp;Wisdom TC Flowable is used four times a year since 2017.</p>

<p>Home garden, sunflower bed, at which carbaryl (Sevin) was used during 2018 growing season.</p>

This soil sample was collected by Jason Ngo. The sample was collected beside train tracks on 249. The location is adjacent to a bayou of water. There are businesses near by such as personal farms, scrap yards, and various buildings and homes. 

<p>Private home garden.</p>

<p>Weather Stone Circle, Katie Leigh Dr.</p>

<p>sample collected 6 yards away from the shore&nbsp;</p>

<p>The location the soil sample was taken from a vegetable farm at 3217 Spring Cypress Rd, Cypress TX 77388. An 8-12 inch deep was dug at this location and about 10g sample was taken. It was stored in a container that protected the sample from UV sunlight. In addition the surrounding temperature was kept at room temperature.</p>

<p>soil sample, texture was very fine</p>

<p>The overall soil sample was dry, but there were some damp soil. The soil was inoculated over a period of 5 weeks. The overall test results were all negative</p>

My soil sample long description - about how and where the sample was taken and any special circumstances

<p>Sample was taken after Hurricane Harvey at Buffalo Bayou. Soil Sample was mostly wet sand/dirt mixture.</p>

<p>Soil sample collected from backyard (side of the house near the main water line). Coordinates: N 29°40.058' W095°35.364' Even though there were signs of pesticide degradation detected in media with Paraoxon (Px) pesticide present, sample did not grow after inoculation into enriched media and overnight incubation. Sample was a false positive.</p>

<p>Soil sample collected from the strawberry field at Froberg's Farm. Sample was taken at 9 am on September 21, 2012. Results over 5 weeks of isolationg and growing the bacteria from the sample. Paraoxon (PX)&amp; Methyl Parathion (MP)are insecticides CSM and SSM are medium. Y = yellow, T= colorless, C = slight yellow and turbid, S=slight yellow and clear, W=turbid white W# is week number PX + CSM = W1-Y, W2-Y, W3-Y, W4-Y, W5-Y PX + SSM = W1-W, W2-T, W3-S, W4-Y, W5-S MP + CSM = W1-W, W2-T, W3-T, W4-T, W5-T MP + SSM = W1-C, W2-C, W3-T, W4-T, W5-T Around 15 colonies of Paraoxon grew on CSM agar plate but no halo was showed on colony.</p>

<p>The soil was collected from farmland in La Porte, Texas where many crops have been grown. I dug a hole approximately 8 inches deep to collect the soil and kept the soil in an amber tube at room temperature to avoid the exposure of sunlight and heat.</p>

<p>My sample was positive for Paraoxon but negative for Methyl Parathion. In the 5 week subculturing leading up to platting there was reason to believe that there was degradation of Paraoxon and Methyl Parathion till week 3 when all yellow color faded from the Methyl Parathion tubes.</p>

<p>Soil collected from a golf course</p>

<p>CSM MP results gave positive results from the beginning about degradation. CSM PX began to show slight degradation around the week 3 of sample transfer. Slight halo effects on the edges of the plate indicates OP hydrolysis has taken place. SSM MP and PX have shown negative results of degradation from the initial stage. In comparison to other members, I did not have any positive results for both SSM Mp/ Px.</p>

<p>Collection of soil sample to test for pesticides, It was collected from my garden where I grew carnations.</p>

<p>The soil sample was collected from garden at home that has frequent pesticides</p>

<p>By the memorial swimming pool, in front of a tree. The dirt was collected from 10 inches deep. Dirt was semi-moist and dark in color.</p>

<p>sample was obtained from golf Course which resulted in dgredgation of OP</p>

<p>Sample was taken from a 3 acre farm that has been in use as farm land since 2012.</p>

<p>This soil sample was collected from the banks of Brays Bayou that recently might have overflowed due to Hurricane Harvey.</p>

The sample was collected in Houston Texas.

the sample was collected from my backyard

Collected 8/29/2018, 7:30 AM. Soil sample was collected nearby bayou and construction site by a biking trail. Soil sample shows negative for Paraoxon under GGM- PX assay.

<p>200 microliter inoculation of week 2's sample into fresh LB media. Samples have been shaken for 1 week in shaker machine (shook for 3 weeks total). SSM(MP) and CSM(MP) are clear, SSM(Px) is slightly yellow, and CSM(Px) is yellow.</p>

<p>Soil collected from 12 inches below the frozen surface.&nbsp; Sample analyzed by Kara Morrison.</p>

<p>The soil obtained was in my parent's garden.</p>

<p><strong>Stenotrophomonas maltophilia CBF10-1&nbsp;</strong></p>

<p>Collected from apple orchard in a private yard. Malathion was used throughout the 2018 growing season.</p>

Alfalfa field at which lorsban was used. 

<p>Private onion patch which was sprayed with&nbsp;malathion during the 2019 growing season.</p>

<p>University garden.</p>

<p>Only herbicides were used here.</p>

<p>Soil Sample from Washington Avenue&nbsp; Coalition/Memorial Park&nbsp; - This soil was very muddy due to the rain on the previous day. I collected the soil next to a park bench. The soil was came in clumps and was easy to break off.&nbsp;</p>

<p>Private home garden at which BiFenthrin was used during the fall of 2019.&nbsp;</p>

A soil sample was taken from the University of Houston Energy Research Park parking lot.

<p>Sample collected in a residential area.</p>

<p>Soil sample was taken from Froberg Farm on August 30th, 2014 at around 11 am. At the time when soil sample was taken, no produce were seen growing on site. The farm land was being prepared for planting new batch of seeds. After the weekly inoculation, an average of 19 colonies were seen growing on the agar plate. The bacteria are tolerant to withstand the toxic environment however they are unable to degrade the organophosphorus compound present in the pesticide</p>

<p>Sample was collected on a day after which it had rained.</p>

<p>Sample Collected near at the gated boardwalk area near the ship since the other side was closed off closer to the waters. Highly polluted area(cigarettes, paper, trash, food, etc)</p>

<p>Got soil from buffalo bayou, by the battle of Texas Historic Site</p>

<p>The sample was taken from cullinan park in January after a stormy day when the soil was still wet.</p>

<p>Sample was collected 8 feet from the fence line.</p>

<p>Paraoxon hydrolyzing bacteria are present. Showed negative results for Methyl Parathion degradation.</p>

<p>Soil Sample was collected from a farm outside Rosenberg. The farm seemed freshly sown because the soil was turned over and some crop seem to have been planted.</p>

<p>Sample taken from a Garden Center which use pesticides in all their plants.</p>

<p>This sample was collected from farmland, which was thought to contain OP hydrolyzing bacteria. The bacteria isolated from the sample, which was collected during the spring 2017 semester, was paraoxon tolerant but not capable of OP hydrolysis.</p>

<p>The sample was a mixture of sand and dirt. It was taken from an area that was affected by flood water. Once grown on a Px plate there was many tolerate bacteria and a few bacteria colonies that degraded the pesticide with soft edges.</p>

<p>I collected a sample that was at the waters edge where many birds had been so the top layer was mostly white bird poop and the lower levels were clay with colorful bands of sand and a little bit of algae that was floating atop it. It smelled like Galveston beach, which is ironic (or not) because Bray's Bayou flows right into Galveston Bay.</p>