Non-Cataloged Sample with POSITIVE Paraoxon NEGATIVE Methyl Parathion

Sample that has not been cataloged in a genomic data repository, with a positive number of Paraoxon bacteria growth and zero Methyl Parathion bacteria growth

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2021 Spring | UH | Wed | Jessica Yang |

  • Approx. 50g of soil sample was collected from the bottom of a hole 12in deep. The soil was placed inside an amber tube and Ziploc bag to protect it from light exposure during transportation to the lab. 
  • Week 0: 1g of soil was added to 6mL of LB media and left to incubate at 30°C, 200rpm for 2 weeks (*1 additional week due to winter storm Uri). 
  • Week 1: 1mL of LB culture was transferred into 4 tubes containing 4mL of CSM/SSM minimal media with Px/MP pesticide for a total of 5mL.

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